LeHavreV1, Main, Exploration, bibRecord, 000357

An intron-containing, heat-inducible stress-70 gene in the millipede Tachypodoiulus niger (Julidae, Diplopoda)

Identifieur interne : 000357 ( Main/Exploration ); précédent : 000356; suivant : 000358

An intron-containing, heat-inducible stress-70 gene in the millipede Tachypodoiulus niger (Julidae, Diplopoda)

Auteurs : Thomas Knigge [France] ; Lutz Bachmann [Norvège] ; Heinz-R. Köhler [Allemagne]

Source :

Cell Stress & Chaperones [ 1355-8145 ] ; 2014.

RBID : PMC:4147066

Descripteurs français

Wicri :
- geographic : Niger.

English descriptors

KwdEn :
- Amino Acid Sequence, Animals, Arthropods (genetics), Arthropods (metabolism), Base Sequence, HSC70 Heat-Shock Proteins (genetics), HSC70 Heat-Shock Proteins (metabolism), Heat-Shock Proteins (metabolism), Heat-Shock Response (genetics), Hot Temperature, Introns, Niger.
MESH :
- chemical , genetics : HSC70 Heat-Shock Proteins.
- geographic : Niger.
- genetics : Arthropods, Heat-Shock Response.
- metabolism : Arthropods, HSC70 Heat-Shock Proteins, Heat-Shock Proteins.
- Amino Acid Sequence, Animals, Base Sequence, Hot Temperature, Introns.

Abstract

The highly conserved part of the nucleotide-binding domain of the hsp70 gene family was amplified from the soil diplopod Tachypodoiulus niger (Julidae, Diplopoda). Genomic DNA yielded 701, 549 and 540 bp sequences, whereas cDNA from heat shocked animals produced only one distinct fragment of 543 bp. The sequences could be classified as a 70 kDa heat shock protein (hsp70), the corresponding 70 kDa heat shock cognate (hsc70) and a glucose-related hsp70 homologue (grp78). Comparisons of genomic and cDNA sequences of hsc70 identified two introns within the consensus sequence. Generally, stress-70 expression levels were low, which hampered successful RT-PCR and subsequent subcloning. Following experimental heat shock, however, the spliced hsc70 was amplified predominantly, instead of its inducible homologue hsp70. This finding suggests that microevolution in this soil-dwelling arthropod is directed towards low constitutive stress-70 levels and that the capacity for stress-70 induction presumably is limited. hsc70, albeit having introns, apparently is inducible and contributes to the stress-70 response.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4147066

DOI: 10.1007/s12192-014-0494-7
PubMed: 24446070
PubMed Central: 4147066

Affiliations:

Le document en format XML

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<series><title level="j">Cell Stress & Chaperones</title>
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<front><div type="abstract" xml:lang="en"><p>The highly conserved part of the nucleotide-binding domain of the <italic>hsp70</italic>
 gene family was amplified from the soil diplopod <italic>Tachypodoiulus niger</italic>
 (Julidae, Diplopoda). Genomic DNA yielded 701, 549 and 540 bp sequences, whereas cDNA from heat shocked animals produced only one distinct fragment of 543 bp. The sequences could be classified as a 70 kDa heat shock protein (<italic>hsp70</italic>
), the corresponding 70 kDa heat shock cognate (<italic>hsc70</italic>
) and a glucose-related <italic>hsp70</italic>
 homologue (<italic>grp78</italic>
). Comparisons of genomic and cDNA sequences of <italic>hsc70</italic>
 identified two introns within the consensus sequence. Generally, <italic>stress-70</italic>
 expression levels were low, which hampered successful RT-PCR and subsequent subcloning. Following experimental heat shock, however, the spliced <italic>hsc70</italic>
 was amplified predominantly, instead of its inducible homologue <italic>hsp70</italic>
. This finding suggests that microevolution in this soil-dwelling arthropod is directed towards low constitutive stress-70 levels and that the capacity for stress-70 induction presumably is limited. <italic>hsc70</italic>
, albeit having introns, apparently is inducible and contributes to the stress-70 response.</p>
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An intron-containing, heat-inducible stress-70 gene in the millipede Tachypodoiulus niger (Julidae, Diplopoda)

An intron-containing, heat-inducible stress-70 gene in the millipede Tachypodoiulus niger (Julidae, Diplopoda)

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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